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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 6 (FZD6)

Anti -Frizzled 6 (FZD6)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH FC
Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
Catalog #F6401-27
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionELISA (Direct): 0.5-1ug/ml The detection limit is ~0.15ng/well.
Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and ~2ng/lane under reducing conditions.
Immunohistochemistry: 5-10ug/ml in fixed cells. Cells were fixed with 4% paraformaldehyde in PBS at RT for 20 mins. Blocked with 0.1% Triton X-100, 1% BSA 10% normal donkey serum in PBS at RT for 45 mins. Cells were then incubated with F6401-27 overnight at 4°C followed by IgG, H&L (Rhodamine) xGt at RT in the dark for 1 hour. Between each step, cells were washed with 0.1% BSA in PBS.
Flow Cytometry: 0.1mg/ml. Use 10ul to label 1-2.5x10e5 cells in a total volume <200ul. The binding of unlabeled primary antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of IgG (FITC) xGt.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1903-03U IgG, H&L (Rhodamine) Pab Rb xGt
I1903-03V IgG, H&L, X-Adsorbed (Rhodamine) Pab Rb xGt
I1904-33A IgG. H&L (Rhodamine) Pab Ch xGt
I1904-33B IgG (H&L), X-adsorbed (Rhodamine) Pab Dk xGt
I1903-30A IgG, H&L, X-Adsorbed (FITC) Pab Rb xGt
I1904-28A IgG, H&L (FITC) Pab Dk xGt
I1904-28E IgG, H&L, X-Adsorbed (FITC) Pab Dk xGt
I1904-33 IgG, H&L (FITC) Pab Ch xGt
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by immunoaffinity chromatography.
Endotoxin<0.5EU/1ug (LAL).
ImmunogenNS0-derived recombinant mouse Frizzled-6 extracellular Cysteine-Rich Domain (CRD).
SpecificityRecognizes mouse Frizzled-6. <1% crossreactivity with recombinant mouse Frizzled-1,-2, -3, -4, -7, -8 and -9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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