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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E B

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.
Catalog #	F6401-27A
Hybridoma	Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled-6 (rmFrizzled-6) extracellular Cysteine-Rich Domain (CRD).
Applications	Suitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended Dilution	Western Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-6. Using a colorimetric detection system, the detection limit for rmFrizzled-6 is ~25ng/lane under non-reducing conditions.
	Direct ELISA: Use at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-6. The detection limit for rmFrizzled-6 extracellular CRD is ~5ng/well.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9j128
Host	Rat
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, with 5% trehalose.
Purity	Purified by Protein G affinity chromatography.
Immunogen	NS0-derived rmFrizzled-6 extracellular Cysteine-Rich Domain (CRD)
Specificity	Recongizes mouse Frizzled-6. Does not crossreact with rhFrizzled-5 or rmFrizzled-1, -2, -3, -4, -5, -7, -8 or -9.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.