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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 7 (FZD7)

Anti -Frizzled 7 (FZD7)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #F6401-32
Applications Direct ELISA, Western blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml.
Western blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-6. The detection limit for rmFrizzled-7 is approximately 1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 1.7-15ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, before the addition of 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rmFrizzled-7, amino acids 33-185
SpecificityRecognizes mouse Frizzled-7 extracellular Cysteine-Rich Domain (CRD). Approximately 15% crossreactivity with rmFrizzled-1, 5% crossreactivity with rmFrizzled-3 and rmFrizzled-6 and less than 2% crossreactivity with rmFrizzled-4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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