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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 7 (FZD7)

Anti -Frizzled 7 (FZD7)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IC FC
Frizzled-7 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Catalog #F6401-32A
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 2.5ug/10e6 cells using retinoic acid-treated D3 mouse embryonic stem cell line.
Immunocytocytochemistry: 8-25ug/ml using immersion fixed HEK293 human embryonic kidney cell line.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9j129
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa33-185 from mouse Frizzled-7 expressed in NS0 cells (Q61090).
SpecificityRecognizes mouse Frizzled-7. Shows no crossreactivity with recombinant mouse Frizzled-1, -3, -4 or -8. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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