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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 7 (FZD7) (Biotin)

Anti -Frizzled 7 (FZD7) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH IC FC
Frizzled-7 belongs to the Frizzled family of seven transmembrane domain receptors. Frizzled proteins function as receptors for Wnt proteins.
Catalog #F6401-32B
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Direct ELISA. Detects Frizzled-7 by Flow Cytometry and Immunohistochemistry on the differentiated embryoid bodies derived from mouse embryonic cell line D3. Flow Cytometry and Immunocytochemistry on human HEK293 cells demonstrate that this antibody crossreacts with human FZD7. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.1mg/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. Binding may be visualized by adding streptavidin conjugated to a fluorochrome.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9j129
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, with BSA (50ug per ug antibody). Labeled with Biotin. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmFrizzled-7 extracellular CRD, aa33-185 (Q61090).
SpecificityRecognizes mouse Frizzled-7. Shows no crossreactivity with rmFrizzled-1, -3, -4 or -8. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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