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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 7 (FZD7)

Anti -Frizzled 7 (FZD7)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Frizzled-7 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Catalog #F6401-32C
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled-7 (rmFrizzled-7) extracellular domain. The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-7. Using a colorimetric detection system, the detection limit for rmFrizzled-7 is ~1ng/lane and ~5ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: Use at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-7. The detection limit for rmFrizzled-7 is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9j130
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, with 5% trehalose.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmFrizzled-7 extracellular domain
SpecificityRecongnizes mouse Frizzled-7. No crossreactivity was observed with rmFrizzled-1, -2, -3, -4, -6 or -8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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