Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Frizzled 7 (FZD7)

Anti -Frizzled 7 (FZD7)


  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Frizzled-7 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Catalog #F6401-32C
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled-7 (rmFrizzled-7) extracellular domain. The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-7. Using a colorimetric detection system, the detection limit for rmFrizzled-7 is ~1ng/lane and ~5ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: Use at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-7. The detection limit for rmFrizzled-7 is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9j130
FormSupplied as a liquid in PBS, pH 7.2, with 5% trehalose.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmFrizzled-7 extracellular domain
SpecificityRecongnizes mouse Frizzled-7. No crossreactivity was observed with rmFrizzled-1, -2, -3, -4, -6 or -8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

External Links