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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 7 (FZD7) (Biotin)

Anti -Frizzled 7 (FZD7) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Frizzled-7 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Catalog #F6401-32D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: Use at 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, BSA. labeled with Biotin. Reconstitute with 1ml sterile TBS, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived recombinant mouse Frizzled-7 corresponding to aa33-185.
SpecificityRecognizes mouse Frizzled-7 extracellular Cysteine-Rich Domain. Species Crossreactivity: recombinant mouse Frizzled-2, ~5%; recombinant mouse Frizzled-3, -4, -5, -6, -8 and -9, <2%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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