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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 8 (FZD8)

Anti -Frizzled 8 (FZD8)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
FZD8 is a Frizzled Receptor that mediates Wnt signaling and is involved in determining vertebrate axis and tissue polarity. FZD8 is expressed during embryonic development in forming and differentiating somites in mice. FZD8 expression has been reported in brain, heart, kidney, pancreas, and skeletal muscle. ESTs have been isolated from embryo, eye, head/neck, heart, kidney, liver/spleen, pancreas, prostate, and testis libraries, as well as many tumor libraries.
Catalog #F6401-37
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml. The detection limit is ~2ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml detects FZD8 in cells and tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with1ml sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived recombinant mouse Frizzled-8 extracellular Cysteine-Rich Domain (CRD)
SpecificityRecognizes recombinant mouse Frizzled-8. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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