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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 8 (FZD8)

Anti -Frizzled 8 (FZD8)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail.
Catalog #F6401-37A
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Use at 1-2ug/ml. Using a colorimetric detection system, the detection limit for rmFrizzled-8 is ~25ng/lane under reducing conditions.
Direct ELISA: Use at 0.5-1.0ug/ml. The detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Endotoxin 1Eu/ug
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9j131
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, 40% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmFrizzled-8 extracellular domain (CRD)
SpecificityRecognizes mouse Frizzled-8. In Direct ELISAs, shows approximately 15% crossreactivity with rmFrizzled-6 and no crossreactivity with rmFrizzled-1, -2, -3, -4, or -7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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