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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 8 (FZD8) (Biotin)

Anti -Frizzled 8 (FZD8) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail.
Catalog #F6401-37B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml. Detection limit for recombinant mouse Frizzled-8 is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute with sterile Tris-buffered saline, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, with BSA. Reconstitute with 1ml of TBS, 0.1% BSA. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived recombinant mouse Frizzled-8 extracellular Cysteine-Rich Domain (CRD).
SpecificityRecognizes mouse Frizzled-8. Less than 5% crossreactivity with recombinant mouse Frizzled-1, Frizzled-2, Frizzled-3, Frizzled-4, Frizzled-6, Frizzled-7 and Frizzled-9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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