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Anti -Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)
Anti -Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| Fibromodulin (FMOD; also FM and collagen-binding 59kD protein) is a secreted, 60-70kD class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-B. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358aa in length (aa19-376). It contains 12 LRRs (Leu-rich repeats) (aa98-376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa88-101. Over aa76-376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin. | | | Catalog # | F7080 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1ug/ml | | Western Blot: 0.3ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | NS0-derived recombinant human Fibromodulin (aa75-376). | | Specificity | Recognizes human Fibromodulin. Species Crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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