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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)

Anti -Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Fibromodulin (FMOD) is a Class II small leucine-rich proteoglycan (SLRP) superfamily member. The ~50-70kD protein contains a sulfated N-terminal complement C1q binding domain (aa19-98) and 12 leucine-rich repeats (aa75-376). A 40kD form is reported in dental tissues. It participates in collagen fibrillogenesis in many tissues and competes with Lumican for collagen binding. It can participate in inflammation by activating complement. In epidermal keratinocytes, it may be located in the cytoplasm. The region of human FMOD used as an immunogen shares 96% aa identity with mouse or rat FMOD.
Catalog #F7080-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human FMOD (aa76-376).
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No9L829
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived, recombinant human FMOD (aa76-376; Accession # NP_002014).
SpecificityRecognizes human Fibromodulin. Species sequence homology: mouse, rat (96%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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