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You are here:Home » Antibodies » Abs to Transcription Factors, Forkhead (FOX) » Anti -FOXP3 (FOXP3, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, SCURFIN, scurfin, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked)

Anti -FOXP3 (FOXP3, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, SCURFIN,
scurfin, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune
dysregulation, polyendocrinopathy, enteropathy, X-linked)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IH
FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
Catalog #F9050-10A
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
ApplicationsSuitable for use in ELISA and Immunohistochemistry (In paraffin embedded human tonsil, stains nuclei of some cells in the interfollicular area). Other applications not tested.
Recommended DilutionELISA: 1:16,000
Immunohistochemistry: 0.3-1ug/ml
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buf
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See F9050-10A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSQRPSRCSNPTPGP, C Terminus
SpecificityRecognizes Human FOXP3/SCURFIN. Species crossreactivity: canine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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