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You are here:Home » Antibodies » Abs to Transcription Factors, Forkhead (FOX) » Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC FC
FOXP3 (a 381aa protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Catalog #F9050-10E
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: Band is seen at ~48kD in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates.
Optimal dilutions to be determined by the researcher.
Positive ControlMouse CD4+CD25+ T cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in Tris-glycine, sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion protein encompassing GST-mouse Foxp3 peptide-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein. Cellular Localization: Nuclear
SpecificityRecognizes mouse FOXP3 (CD4+CD25+T cells). Does not react with human Jurkat or mouse CD4+CD25-T cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMGC141961, MGC141963


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