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You are here:Home » Antibodies » Abs to Transcription Factors, Forkhead (FOX) » Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis.
FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Catalog #F9050-10K
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:50. Tested on mouse thymus from an influenza-infected mouse or thymus from healthy mouse.
Western Blot: 1:1000-1:5000 detects a band at~47.2kD
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide corresponding to a region between aa400-431 at the C-terminal region of human FOXP3 (Q9BZS1)
SpecificityRecognizes human FOXP3. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMGC141961, MGC141963


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