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You are here:Home » Antibodies » Abs to Transcription Factors, Forkhead (FOX) » Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

Anti -FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B IH IC
FoxP3 is a 48kD forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis. FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.
Catalog #F9050-12
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsELISA (Direct and indirect): 1:2000-1:10,000
Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is TBST, 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at RT.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6D401
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservative added.
PurityPurified
ImmunogenRecombinant protein corresponding to mouse FOXP3 (forkhead box protein P3).
SpecificityRecognizes both natural and recombinant mouse FOXP3. Detects a band of ~46-49kD by Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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