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You are here:Home » Molecular Biology » Growth Factors-Follistatin » Follistatin, Recombinant, Human (FST, FS, Activin-binding Protein)

Follistatin, Recombinant, Human (FST, FS, Activin-binding Protein)

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Specifications

Follistatin (FS), a glycoprotein, plays an important role in cell growth and differentiation through the neutralization of the biological activities of activins. Follistatin (FST) is a monomeric activin-binding and neutralization protein that has at least three isoforms in human tissues and fluids. The full-length FS315 protein has an acidic 26-residue C-terminal tail that is not present in the shortest form, FS288, due to alternative splicing. An intermediate form, FS303, was identified in follicular fluid that is presumably derived by proteolytic processing of this tail domain. Interestingly, the biochemistry of each of these three isoforms is distinct, including their ability to bind to cell surface proteoglycans, an activity that ranks in the order FS288 > FS303 > FS315.
Recombinant Human Follistatin produced in E. coli is a single, non-glycosylated polypeptide chain containing 288 amino acids and having a total molecular mass of 31.5kD.
Catalog #F9060-05
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Gly-Asn-Cys-Trp-Leu.
Biological ActivityRecombinant Follistatin is fully biologically active when compared to standard.
The activity is determined by the ability to neutralize Activin A inhibitory effect of mouse MPC-11 cells. The expected ED50 is 0.1-0.4ug/ml in the presence of 7.5ng/ml Activin A.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight31.5kD
SourceE. coli
Purity~95% as determined by RP-HPLC and SDS-PAGE
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from SBS. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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