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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Fz5 (FZD5, Frizzled Homolog 5 (Drosophila), C2orf31, Chromosome 2 Open Reading Frame 31, DKFZp434E2135, DKFZP434E2135, Frizzled-5, Frizzled 5 precursor, Frizzled-5 precursor, Fz-5, FzE5, hFz5, Hfz5, HFZ5, MGC129692, Uncharacterized Protein C2orf31)

Anti -Fz5 (FZD5, Frizzled Homolog 5 (Drosophila), C2orf31, Chromosome 2 Open Reading
Frame 31, DKFZp434E2135, DKFZP434E2135, Frizzled-5, Frizzled 5 precursor, Frizzled-5
precursor, Fz-5, FzE5, hFz5, Hfz5, HFZ5, MGC129692, Uncharacterized Protein C2orf31)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), beta- and gamma- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
Catalog #F9900
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000. Lysates from mouse liver, C2C12, Jurkat and HUVEC cells were resolved by electrophoresis, transferred to PVDF and probed with F9900.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Positive ControlMouse liver, C2C12, Jurkat and HUVEC cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, 30% glycerol.
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic peptide corresponding to aa564585, C-TGRTPPGPAATYHKQVSLSHV, of human Fz5 conjugated to KLH (NP_003459). Epitope: C-terminus.
SpecificityRecognizes human Fz5 at ~62kD. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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