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You are here:Home » Molecular Biology » MB-Carbohydrates, Glycoproteins » G6b, Recombinant, Human, Fc Chimera (C6orf25, chromosome 6 open reading frame 25MGC142279, MGC142281, NG31, Protein G6b)

G6b, Recombinant, Human, Fc Chimera (C6orf25, chromosome 6 open reading frame 25MGC142279,
MGC142281, NG31, Protein G6b)

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Specifications

G6b is a t ype I transmembrane glycoprotein in the immunoglobul in superfamily that is encoded within the human class III major histocompatibility complex (1 ­ 3). It is expressed mainly on platelets, and has also been detected on megakaryocytes and the human cell line K562 (2 ­ 5). Crosslinking of G6b inhibits agonist­stimulated platelet aggregation and activation (5). The 241 amino acid (aa) human G6b isotype B (G6b­B) contains a 17 aa signal sequence, a 125 aa extracellular domain with one V­type Ig ­like domain and one N­linked glycosylation site, a 21 aa transmembrane sequence and a 78 aa cytoplasmic sequence with two ITIM inhibitory motifs and a SH3 bindingsite (1, 2). Phosphorylation at Y211 within the first ITIM is required for interaction with SHP adapt or/ tyrosine phosphatase proteins (2, 3). Of several described isoforms, all are identical within aa 22­136, which includes most of the extracellular domain. One contains an alternate signal sequence, while at least two lack a transmembrane sequence and are secreted (2). At least two others, including G6b­A, express alternate C­t ermini that lack the ITIM motifs. Of the known isoforms, G6b­A and G6b­B predominate in platelets. It is possible that G6b­A may be paired with and control inhibition by G6b­B (4). Heparin interacts with the G6b extracellular domain with high­affinity and may contribute to G6b activity or adhesion to extracellular matrix (6).
Catalog #G0900-25
Source Chinese Hamster Ovary cell line, CHO-derived Human G6b (Asn18 Gln142), Accession # O95866, IEGRMD, Human IgG1, (Pro100 Lys330).
Predicted Molecular Mass 40.0kD (monomer) SDS-PAGE under reducing conditions.
Activity Measured by its binding ability in a functional ELISA. When 1ug/ml of rhG6b/Fc is captured by a goat anti-human IgG Fc coated plate, the concentration of biotinylated heparin that produces 50% of the optimal binding response is found to be approximately 10-40ng/ml.
Endotoxin 1.0 EU per 1 ug of the protein by the LAL method.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability12 months from date of receipt, 20 to 70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
months, 20 to 70°C under sterile conditions after reconstitution.
Avoid repeated freeze thaw cycles.
SourceSource Chinese Hamster Ovary cell line
Purity95% by SDS­PAGE under reducing conditions and visualized by silver stain.
Concentration~100ug/ml
FormLyophilized from a 0.2um filtered solution in PBS. Reconstitute at 100ug/ml in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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