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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a,

Anti -G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine
N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3,
Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8,
NG36, NG36/G9a,

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1.
Catalog #G1009-02A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.25-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenLinear peptide corresponding to G9a (KLH). Cellular Localization: Nucleus.
SpecificityRecognizes human G9a. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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