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You are here:Home » Antibodies » Abs to Amino Acids » Anti -GABA (Gamma-aminobutyrIc acid)

Anti -GABA (Gamma-aminobutyrIc acid)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
Both neural and neuroendocrine cells are able to synthesize a variety of peptides as well as amino acids that can function either as inhibitory or stimulatory substances in neurotransmission. Thusfar, mostly polyclonal antibodies are available for many of them. In the central nervous system GABA is an early neurotransmitter, which can be detected in the developing nervous system. GABA functions as a neurotransmitter also in the peripheral nervous system. Most studies thusfar published on immunolocalization of GABA containing neurons have been made with rabbit polyclonal antibodies. Clearly, there is an increasing need for monoclonal antibodies against neurotransmitters that would enable double immunolocalization studies.
Catalog #G1015-02A
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:500-1: 20,000.
Immunohistochemistry: 1:500-1: 20,000. Works well on frozen sections if perfusion fixed. Paraffin sections require glutaraldehyde in the fixative.
Optimal dilutions to be determined by the researcher.
HybridomaFusion between myeloma cells and Balb/c spleen cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5G10
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenGABA-coupled to BSA with glutaraldehyde
SpecificityRecognizes GABA. Does not react with other amino acid-BSA conjugates with the exception of a weak reaction against b-alanine BSA-conjugate at the highest concentrations used.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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