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You are here:Home » Antibodies » Abs to Receptors » Anti -GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA1)

Anti -GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit,
GABRA1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The neurotransmitter GABA binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. The GABAA receptor is a multiprotein complex that acts as a ligand-gated chloride channel. There are at least 6 different isoforms of the alpha subunit. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.
Catalog #G1015-06
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:10,000 detects the GABA Receptor A, alpha1 subunit in 10ug of rat brain microsomal preparation. tissue lysate. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 5ul immunoprecipitates the GABA Receptor A, alpha1 subunit in rat brain microsomal preparation RIPA lysate.
Immunohistochemistry (Paraffin): 1:500 detects the GABA Receptor A, alpha1 subunit in rat hippocampus tissue. Requires HIER.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenLinear peptide corresponding to the topological domain of rat GABA Recptor A, alpha1, conjugated to KLH. (NP_899155). Species Sequence Homology: human, 94%
SpecificityRecognizes rat GABA Receptor A, alpha 1 at ~51kD. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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