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You are here:Home » Antibodies » Abs to Transport Proteins » Anti -GABA Transporter 3 (GAT-3 (Gamma aminobutyrIc acid)

Anti -GABA Transporter 3 (GAT-3 (Gamma aminobutyrIc acid)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOH- termini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.
Catalog #G1015-76
ApplicationsSuitable for use in ELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000. Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid with 0.05% sodium azide. Reconstitute with 100ul PBS, pH 7.4.
PuritySerum
ImmunogenA 15 AA synthetic peptide near the C-terminus of rat GAT3 (1) (KLH).
SpecificityRecognizes rat GAT3. Species sequence homology: mouse (100%) and human (92%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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