| GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. |
|
| GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. |
|
| Catalog # | G1016 |
| Applications | Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.5-2ug/ml detected the GABA A Receptor beta 2,3 subunits from 20ug of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G1016 (2ug/ml). Proteins were visualized using a IgG (HRP) GtxMo and ECL. |
| Immunoprecipitation: 4ug immunoprecipated the GABA A Receptor beta 2,3 subunits from 500ug of rat brain microsomal preparation. Due to interference of the IgG heavy chain, the immunoprecipitated GABA A reseptor complex was confirmed using G1015-06. |
| Immunohistochemistry: Detects GABA A Receptor beta 2,3 subunits in paraffin embedded and frozen rat brain sections fixed with 4% paraformaldehyde or 1% glutaraldehyde and permeabilized with Triton X-100. |
| Optimal dilutions to be determined by the researcher. |
| Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 0.T.52 |
| Host | Mouse |
| Source | Bovine |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Affinity-purified GABAA receptor isolated from bovine brain. |
| Specificity | Recognizes bovine GABAA Receptor b2 (55kD) and b3 (57kD) subunits. Species Crossreactivity: human and rat. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
