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You are here:Home » Antibodies » Antibodies-Enzymes, Glycosidase » Anti - -Galactosidase

Anti - -Galactosidase


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Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IC FC IF
Proteins can be tagged using recombinant DNA techniques to allow for their purification and or detection in different cell systems. b-galactosidase has been used extensively as a fusion partner since b-gal fusion proteins can also be detected by quantitation b-galactosidase enzymatic activity.
Epitope tags are useful for the labeling and detection of proteins using Western Blot, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteinís biochemical properties.
Catalog #G1041-02
Apparent Molecular WeightThe apparent molecular weight is related to the fusion protein.
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence/Immunocytochemistry Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (IC): 1:3200
Flow Cytometry: 1:7000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3A11
ConcentrationNot Determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
ImmunogenRecombinant beta galactosidase protein.
SpecificityRecognizes beta-Galactosidase. Detects beta-galactosidase fusion proteins overexpressed in cells. Does not recognize any other overexpressed protein. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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