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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli) (Beta-gal, b0344, JW0335, Lactase, LacZ) (HRP)

Anti - -Galactosidase (E. coli) (Beta-gal, b0344, JW0335, Lactase, LacZ) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116kD each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
Catalog #G1041-02D3
ApplicationsSuitable for use in ELISA, Immunohistochemistry (frozen and paraffin), and Western Blot. Other applications not tested.
Recommended Dilutionimmunohistochemistry (frozen): 1:50-1:200
immunohistochemistry (paraffin): 1:50-1:200
ELISA: 1:200-1:1500
Western Blot: 1:50-1:300
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% thiomersal, 0.001% gentamicin sulphate, 0.1% BSA. Labeled with horseradish peroxidase (HRP).
PurityPurified by Ion Exchange chromatography.
ImmunogenBeta-galactosidase prepared from E. coli.
SpecificityRecognizes Beta-galactosidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.2.1.23


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