Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » -Galactosidase, E. coli (Beta-gal, b0344, JW0335, Lactase, LacZ)

-Galactosidase, E. coli (Beta-gal, b0344, JW0335, Lactase, LacZ)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

E. coli Beta-Galactosidase is an inducible tetrameric enzyme coded by the lac Z gene of the lac operon that is often used as a reporter to assess the efficiency of transfection. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Catalog #G1041-02J
SourceBacterial
Molecular Weight~540kD
Activity> 600 U/mg proteinOne unit hydrolyses 1 µmole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37ºC.
ApplicationsSuitable for use in Functional Assays. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
FormSupplied as a lyophilized powder in 10mM potassium phosphate, 74% sucrose. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.2.1.23


External Links