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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » -Galactosidase (Beta-galactosidase, Beta-gal) (Biotin)

-Galactosidase (Beta-galactosidase, Beta-gal) (Biotin)

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Specifications

Biotinylated b-galactosidase is prepared from an enzyme of exceptionally high specific activity isolated from a special strain of E. coli. Unlike mammalian b-galactosidase, the activity of this E. coli enzyme is optimal at an alkaline pH, enabling it to be used free of interference from endogenous enzyme activity. For many applications, Avidin D followed by biotinylated b-galactosidase affords a highly sensitive system which can be used in a variety of enzyme immunoassays, including screening for monoclonal antibodies. One unit is 1 µmole o-nitrophenyl-b-D-galacto-pyranoside hydrolyzed per min. at 37 °C, pH 7.3.
Catalog #G1041-02K
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
ActivityAs Reported
Unit DefinitionOne unit = 1umole of o-nitrophenol liberated from o-nitrophenyl-beta-D-galactopyranoside per minute at 37ºC (pH 7.3).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
ConcentrationNot Determined
FormSupplied as a powder from 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.2.1.23, b-gal


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