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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli)

Anti - -Galactosidase (E. coli)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Anti-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein.
Catalog #G1041-04
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot (native gels): 1:1000-1:2000.
ELISA: 1:1000-10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H21
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
PurityAscites
ImmunogenRecombinant purified E. coli beta-galactosidase
SpecificityRecognizes B-galactosidase protein (~16kD). In ELISA, reacts with the soluble and native enzyme coated on the plate. Does not detect the denatured or reduced proteins in Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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