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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » -Galactosidase (E. coli), Recombinant, ELISA Control

-Galactosidase (E. coli), Recombinant, ELISA Control

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Specifications

Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enable a selective identification and purification of the protein of interest. Eukaryotic genes are often cloned into E. coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3). Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation. Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6). Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein.
Catalog #G1041-05H
SourceRecombinant purified E. coli Beta-galactosidase protein
ApplicationsSuitable for use in ELISA as a control. This preparation is not biologically active. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Other applications not tested.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
PurityPurified
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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