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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli)

Anti - -Galactosidase (E. coli)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IP IH
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Catalog #G1041-05P
Cellular Localization Cytoplasmic
Positive Control 1.0g of E.coli in a standard sandwich ELISA.
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.
Recommended DilutionELISA: 1:4000-1:6000
Immunohistochemistry: 1:500-1:3000
Immunoprecipitation: 1:500-1:5000
Western Blot: 1:2000-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceE. coli
ConcentrationNot determined
FormSupplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, 50% glycerol.
PurityPurified
ImmunogenRecombinant full length protein (E. coli).
SpecificitySpecies Crossreactivity: Crossreacts with E. coli.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.2.1.23, b-gal


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