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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli)

Anti - -Galactosidase (E. coli)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Catalog #G1041-08
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Protein Purification. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Use Protein G): 2ug/mg protein lysate
Western Blot: 1ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.
Positive ControlPurified recombinant b-galactosidase protein or transfected cell lines expressing b-galactosidase-tagged proteins.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F184
HostMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G1041-08X
PurityPurified by Protein G affinity chromatography.
ImmunogenE.coli b-galactosidase. MW
Depends upon the localization of the parent protein tagged with b-galactosidase.
SpecificityMay be used for detection of b-galactosidase expressed by the E.coli lacZ gene encoded in many cloned gene sequences and serves as an indicator for fusion proteins encoded by an inserted DNA sequence. Species Crossreactivity: Proteins from all species which are tagged with b-galactosidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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