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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli) (BSA & Azide Free)

Anti - -Galactosidase (E. coli) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Catalog #G1041-08X
MW of Antigen 116kD
Epitope Not determined
Applications and Recommended DilutionsImmunoprecipitation (Use Protein G) (Ab 2ug/mg protein lysate)
Protein purification
Western Blotting (Ab 1ug/ml for 2hrs at RT) The optimal dilution for a specific application should be determined by the investigator.
Positive Control Purified recombinant b-galactosidase protein or transfected cell lines expressing b-galactosidase-tagged proteins.
Cellular Localization Depends upon the localization of the parent protein tagged with b-galactosidase.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F184
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See G1041-08
PurityPurified by Protein G affinity chromatography.
ImmunogenE.Coli b-galactosidase
SpecificityMay be used for detection of b-galactosidase expressed by the E.coli lacZ gene encoded in many cloned gene sequences and serves as an indicator for fusion proteins encoded by an inserted DNA sequence. Species Crossreactivity: Proteins from all species which are tagged with b-galactosidase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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