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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti - -Galactosidase (E. coli) (Biotin)

Anti - -Galactosidase (E. coli) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH IF
Catalog #G1041-42
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorecscence, Immunomicroscopy. Other applications have not been tested.
Recommended DilutionELISA: 1:10,000- 1:50,000.
Western Blot: 1:2,000- 1:10,000.
Immunohistochemistry : 1:1,000- 1:5,000. Frozen tissue sections, paraffin-embedded tissues, and 4% paraformaldehyde-fixed tissues.
Immunofluorescence: 1:5,000 -1: 10,000.
Optimal dilutions to be determined by the researcher.
LabelBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio10-20 BAC molecules per rabbit IgG molecule.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 10mg/ml BSA, 40% glycerol. Labeled with biotinamidocaproate n-hydroxysuccinimide ester (BAC).
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenAffinity purified beta-galactosidase (E. coli).
SpecificityRecognizes a 464kD band representing E. coli beta-Galactosidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti biotin, anti rabbit serum and purified and partially purified -Galactosidase (E. coli).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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