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You are here:Home » Antibodies » Antibodies-Enzymes, Glycosidase » Anti - -Galactosidase (E. coli) (Biotin)

Anti - -Galactosidase (E. coli) (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH IF
Catalog #G1041-42A
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorecscence, Immunomicroscopy. Other applications have not been tested.
Recommended DilutionELISA: 1:10,000- 1:50,000.
Western Blot: 1:2,000- 1:10,000.
Immunohistochemistry : 1:1,000- 1:5,000. Frozen tissue sections, paraffin-embedded tissues, and 4% paraformaldehyde-fixed tissues.
Immunofluorescence: 1:5,000 -1: 10,000.
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio10-20 Biotin molecules per rabbit IgG molecule.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide Labeled with Biotin.
PurityPurified by delipidation, salt fractionation and ion exchange chromatography.
ImmunogenAffinity purified beta-galactosidase (E. coli).
SpecificityRecognizes a 464kD band representing E. coli beta-Galactosidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti biotin, anti rabbit serum and purified and partially purified -Galactosidase (E. coli).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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