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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » -Galactosidase 1-3, Recombinant, X. campestris (Beta-galactosidase-1-like protein 3, FLJ9023)

-Galactosidase 1-3, Recombinant, X. campestris (Beta-galactosidase-1-like protein 3,
FLJ9023)

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Specifications

The majority of secreted and membrane proteins are glycosylated (1, 2) . Proper glycosylation might be critical for protein folding and biological functions (3, 4). Galactoside is an essential sugar commonly found on various glycan conjugates and galactosidases are among the earliest enzymes to be studied (5). 1­3 Galactosidase from Xanthomanas capestris is a useful tool for removing 1­3 linked galactosides from the non­reducing terminus of glycoconjugates ( 6, 7).
Catalog #G1041-48
Source E. coli-derived Ser25 Glu613 with an N-terminal Met and 6 His
tag, Accession # NP_638243
N-terminal Sequence Analysis Met
Predicted Molecular Mass 67kD (monomer)
SDSP-AGE: 63kD, reducing conditions
ActivityMeasured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl Dgalactopyranoside. The specific activity is 14,000 pmol/min/ug, as measured under the described conditions.
Endotoxin 1.0 EU per 1ug of the protein by the LAL method.
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and Stability12 months from date of receipt, -20 to -70°C as supplied.
1 month, 4°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Purity95% by SDS­PAGE under reducing conditions and visualized by silver stain.
ConcentrationAs reported
FormSupplied as a 0.2um sterile-filtered solution in Tris and NaCl.
SpecificityRecognizes human Galactosidase 1-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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