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You are here:Home » Molecular Biology » MB-Lectins » Galectin-1, Recombinant, Human (GAL1, LGALS1, GAL-1, Lectin galactoside-binding soluble 1, Beta-galactoside- binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14kD lectin, HPL, HBL, Putative MAPK-activating protein PM12, GBP, DKFZ

Galectin-1, Recombinant, Human (GAL1, LGALS1, GAL-1, Lectin galactoside-binding soluble 1,
Beta-galactoside- binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1,
Galaptin, 14kD lectin, HPL, HBL, Putative MAPK-activating protein PM12, GBP, DKFZ

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Specifications

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 is an autocrine negative growth factor that regulates cell proliferation. Galectin-1 regulates cell apoptosis and cell differentiation. Galectin-1 binds CD45, CD3 and CD4 & inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of lyn kinase. Galectin-1 and its ligands are one of the master regulators of immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host–pathogen interactions. Galectin-1 expression or overexpression in tumors and/or the tissue surrounding them must be considered as a sign of the malignant tumor progression that is often related to the long-range dissemination of tumoral cells (metastasis), to their dissemination into the surrounding normal tissue, and to tumor immune-escape. Galectin-1 in its oxidized form plays a number of important roles in the regeneration of the central nervous system after injury. The targeted overexpression (or delivery) of Galectin-1 should be considered as a method of choice for the treatment of some kinds of inflammation-related diseases, neurodegenerative pathologies and muscular dystrophies. In contrast, the targeted inhibition of Galectin-1 expression is what should be developed for therapeutic applications against cancer progression. Galectin-1 is thus a promising molecular target for the development of new and original therapeutic tools.
Catalog #G1044-47B
Human, recombinant Galectin-1 is produced in E. coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 14.5kD.
Accession NumberNP_005558
Amino acid sequenceACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD
Biological ActivityHuman Galectin-1 is a chemoattractant for human blood monocytes. ED50 range is from 0.05-3ug/ml.
Protein ContentUV spectroscopy at 280nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
ApplicationsSuitable for use in Western Blot, ELISA as an Antigen, Ligand, or Assay Standard. Other applications not tested.
Important Note This recombinant enzyme has NOT been tested with regard to biological activity.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1 % HSA or BSA ,aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight14.5kD
SourceE. coli Accession Number: NP_005558
PurityChromatographically purified ~95% by SDS-PAGE, RP-HPLC.
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 10mM sodium phosphate buffer, pH-7.5. Reconstitute with sterile ddH2O, 0.1HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solution
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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