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You are here:Home » Antibodies » Abs to Lectins » Anti -Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2) (Biotin)

Anti -Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin
3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein,
Lectin Galactose Binding Soluble 3, LGALS3, MAC2) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Galectin 3 is one of the more extensively studied member of this family and is a 30kD protein known by various other names including eBP (IgE binding protein), Mac-2, CBP-30 and L-29. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. This antibody has been shown to potentiate the binding of Galectin 3 to IgG. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an important region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas (an apoptosis inducer) treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells.
Catalog #G1044-50B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hGalectin-3 by direct ELISA (using 100ul/well antibody solution) this antibody can be used at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2ng/well of recombinant hGalectin-3.
Western Blot: To detect hGalectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-3 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA, 40-50% glycerol.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from a sterile-filtered PBS solution. Conjugated to Biotin.
PurityPurified by affinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Galectin-3
SpecificityRecognizes human Galectin-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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