Anti -Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal
protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation. | | | Catalog # | G1044-71 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20. | | Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 9H126 | | Host | Mouse | | Source | Human | | Concentration | ~500ug/ml | | Form | Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. | | Purity | Purified by Protein G immunoaffinity chromatography. | | Immunogen | Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14; aa 1-139; Accession# Q8TCE9). | | Specificity | Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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