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You are here:Home » Antibodies » Antibodies-Enzymes, Synthetase » Anti -Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)

Anti -Gamma Glutamylcysteine Synthetase (GCS, Glutamate-Cysteine Ligase)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.
Catalog #G2002
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml for 2hrs at RT
Immunoprecipitation: 10ug/mg protein lysate. Denatured verified. Use Protein A.
Optimal dilution determined by the researcher.
Positive ControlJurkat, MAD109, PC12 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa295-313 of rat GCLC (heavy subunit of GCS). Cellular Localization: Cytoplasmic
SpecificityRecognizes the heavy subunit of rat Gamma Glutamylcysteine Synthetase at ~73kD. Species Crossreactivity: human, monkey and mouse. Crossreacts weakly with bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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