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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Gangliosides play vital roles in various cellular functions such as cell-cell adhesion, communication, and cell-substrate interactions. The divers sugar groups are generated by different types of O-substitutions, usually O-acetyl esters, of the parent molecules. D1.1 is raised against B49 cell line, and it specifically recognizes O-acetyl gangliosides. O-acetyl ganglioside is found in developing rat embryonic neuroectoderm and many human melanoma cells. In developing rat cerebellum, the O-acetyl ganglioside antigen was localized by immunofluorescence to germinal cells of the external granule layer. The D1.1 antigen is a marker for germinal cells, and the acquisition and subsequent loss of it is an aspect of the biochemical maturation of nervous and glial cells.
Catalog #	G2005-50A
Applications	Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Frozen): 2-5ug/ml
	Immunofluorescence: 2-5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	6A88
Host	Mouse
Source	Rat
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Purity	Purified by Protein A chromatography
Immunogen	B49 cells
Specificity	Recognizes O-acetyl gangliosides in rat brain tumor and most human malignant melanoma tumors. Does not crossreact with normal human skin melanocytes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.