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You are here:Home » Antibodies » Abs to Enzymes, Dehydrogenase » Anti -GAPDHS (Glyceraldehyde-3-phosphate Dehydrogenase, Testis-specific, Spermatogenic Cell-specific Glyceraldehyde 3-phosphate Dehydrogenase 2, GAPDH-2, Spermatogenic Glyceraldehyde-3-phosphate Dehydrogenase, GAPD2, GAPDH2, GAPDS, HSD35, HSD-35)

Anti -GAPDHS (Glyceraldehyde-3-phosphate Dehydrogenase, Testis-specific, Spermatogenic
Cell-specific Glyceraldehyde 3-phosphate Dehydrogenase 2, GAPDH-2, Spermatogenic
Glyceraldehyde-3-phosphate Dehydrogenase, GAPD2, GAPDH2, GAPDS, HSD35, HSD-35)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IH
GAPDS is a 40-44kD member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56kD in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408aa in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa223-226. One splice variant shows a 9aa insertion after Ser220. Over aa75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.
Catalog #G2010-10C
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa75-222 from human GAPDHS expressed in E. coli.
SpecificityRecognizes human GAPDHS. Species sequence homology: recombinant human GAPDH and recombinant mouse GAPDH (~1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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