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You are here:Home » Antibodies » Abs to Serum Proteins » Anti -Gastric Inhibitory Peptide (GIP)

Anti -Gastric Inhibitory Peptide (GIP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum RIA
Catalog #G2019
ApplicationsSuitable for use in RIA only. Other applications not tested.
Recommended DilutionRIA: Reconstitute product to 1ml using a proteinaceous physiological buffer. Divide into 100ul aliquots. When required, dilute each 100ul aliquiot to 2.5ml, using 100ul per test.-
Optimal dilutions to be determined by the researcher.
Recommended Assay ConditionsAssay Volume: 1ml
Plasma Volume: 250ul
Label: Monoiodinated GIP 125I = 1fmol/tube
Incubation: 5days at 4°C
Separation: Addition of 4mg charcoal suspension(Norit GSX) in buffer per tube. Mix. Incubate for 10 minutes at 4°C. Separate by centrifugation .
Sensitivity: 1fmol/assay tube
Detection Limit: 95% confidence level
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourcePorcine
ConcentrationNot determined
FormSupplied as a lyophilized powder in 0.05M Barbitone.
PurityNeat Serum
ImmunogenPorcine GIP conjugated to haemocyanin.
SpecificityRecognizes C-Terminal aa15-43. No reactivity with N-Terminal fragments. Slightly crossreactive with pancreatic glucagon. Detects ~20% Big GIP and ~80% normal GIP in fasting plasma, incomplete reaction (~30%) with human GIP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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