| Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. GIP, Gastric inhibitory polypeptide, also known as glucose-dependent insulinotropic polypeptide (GIP), is a 42-amino acid hormone (chr 17q21.3) that stimulates insulin secretion in the presence of glucose. GIP is derived by proteolytic processing of a 153-residue precursor, preproGIP; it is a member of a family of structurally related hormones that includes secretin, glucagon, vasoactive intestinal peptide, and growth hormone-releasing factor. |
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| Catalog # | G2019-02 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1-10ug/ml using Chemiluminescence technique. |
| ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Control Peptide | G2019-02B: Gastric Inhibitory Peptide, Human, Control Peptide (GIP) |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 10aa at the middle region, cytoplasmic domain of human GIP. Species Sequence Homology: mouse, porcine and rat - 90%. |
| Specificity | Recognizes human Gastric Inhibitory Peptide. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
