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You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -Gastric Inhibitory Polypeptide (GIP)

Anti -Gastric Inhibitory Polypeptide (GIP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Human gastric inhibitory polypeptide (GIP) is a 42aa peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa. GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from ~50% identity for glucagon to 30% identity for GLP-2).
Catalog #G2019F
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000; Using glutaraldehyde-linked GIP-OA (molar ratio 4:1) coated at 1ug/ml (or GIP coated directly at 2ug/ml). G2019F binds free GIP in solution.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/mIL-6 myeloma cells with spleen cells from NMRI x Balb/c mice.
Culture MediumRPMI 1640 with 10% fetal calf serum
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F106
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Also available without azide. See G2019F1.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenSynthetic human gastric inhibitory polypeptide
SpecificityRecognizes human Gastric Inhibitory Polypeptide (GIP). Binds free GIP in solution. Crossreacts up to 10% with PACAP and human GLP-2. < 0.1% crossreactivity with glucagon, GLP-1 and VIP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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