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You are here:Home » Antibodies » Antibodies-Transcription Factors, Zinc (Ring) Finger » Anti -GATA4, phosphorylated (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4)

Anti -GATA4, phosphorylated (Ser105) (GATA Binding Factor 4, GATA Binding Protein 4)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
GATA-4 is a 46kD member of the GATA family of zinc finger-containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling; it plays a critical role in regulating basal and agonist or stress-induced gene expression in cardiac and smooth muscle cell types. GATA-4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA-4 is phosphorylated in response to agonist stimulation through MEK1-ERK1/2 and weakly through JNK or p38 MAPKs.
Catalog #G2021-51
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Controls UsedPhenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA-4.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from a region of human GATA-4 that contains serine 105.
SpecificityRecognizes mouse GATA-4 when phosphorylated at Ser105. Species sequence homology: human, rat and chicken (100%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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