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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -GATA4 (GATA Binding Protein 4, GATA-binding Factor 4, Transcription Factor GATA-4)

Anti -GATA4 (GATA Binding Protein 4, GATA-binding Factor 4, Transcription Factor GATA-4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA
sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and
morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4
interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to
regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding
domains and migrates at 56kD in SDS-PAGE gels. Human GATA-4 share 93%, 93% and
92% aa sequence identity with porcine, rat and mouse GATA-4, respectively.
Catalog #G2021-51C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H128
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human GATA-4, aa27-212; P43694 (E. coli).
SpecificityRecognizes human GATA-4 at ~56kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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