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You are here:Home » Antibodies » Abs to Enzymes, Synthetase » Anti -GCLM (Glutamate-cysteine Ligase Modifier Subunit, Gamma-glutamylcysteine Synthetase Regulatory Subunit, Gamma-ECS Regulatory Subunit, GCS Light Chain, Glutamate-cysteine Ligase Regulatory Subunit, GLCLR) (Azide Free)

Anti -GCLM (Glutamate-cysteine Ligase Modifier Subunit, Gamma-glutamylcysteine Synthetase
Regulatory Subunit, Gamma-ECS Regulatory Subunit, GCS Light Chain, Glutamate-cysteine
Ligase Regulatory Subunit, GLCLR) (Azide Free)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Catalog #G2023-39
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10C341
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to a portion of amino acids within aa1-275 of human glutamate-cysteine ligase regulatory subunit coupled to glutathione S transferase (GST).
SpecificityRecognizes human GCLM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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