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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IC

The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005).
Catalog #	G2023-60
Applications	Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions	Immunocytochemistry: 5-20ug/ml
	Western Blot: 0.5-2.5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9H238
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Membrane prepared from testicular seminoma.
Specificity	Recognizes human GCTM-5 antigen. The epitope recognized by GCTM-5 is found on a 50kD protein present on the surface of these cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.