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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -GFAP (Glial Fibrillary Acidic Protein, Astrocyte)

Anti -GFAP (Glial Fibrillary Acidic Protein, Astrocyte)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
GFAP is a 50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons. This antibody detects all three isoforms.
Catalog #G2032-25H
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml
Immunohistochemistry (Formali fixed paraffin, formalin/acetone fized tissues): 0.5-1ug/ml
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG
Clone No9G362
HostMouse
SourcePorcine
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with 1ml sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenGFAP from porcine spinal cord.
SpecificityRecognizes porcine GFAP at ~50kD. Species Crossreactivity: human, mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAstrocyte, Glial fibrillary acidic protein,


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